Table 1.

Gradient program for LC of the LC/MS system. Eluent A was 0.1% formic acid solution, and eluent B was 95% acetonitrile in 0.1% formic acid solution. The flow rate was kept constant at 0.5 mL/min.

Time (min)	Eluent A (%)	Eluent B (%)
1	100.0	0.0
8	0.0	100.0
10	0.0	100.0
12	100.0	0.0