Figure 1.

The effect of hydrogel properties on differentiation of EBs. (A,B) Phase contrast images of EBs. And cell proliferation was measured by using Alamarblue assay at same time point. (C) Expression of vasculogenic (PECAM1, Tie2) and cardiogenic (alpha-MHC and Gata4) in EBs after 6 days, and in EBs before encapsulation (left line). (D) Immunostaining PECAM1 (green) (scanning confocal microscopy) in EBs encapsulated in 3 wt % GelMA (left lane) and 10 wt % PEG (right lane) after 7 days culture with nuclear co-staining (PI, red) [40]. (Reproduced with permission from Qi H et al. Adv Mater; published by John Wiley and Sons, 2010).