Table 1.
Primers used in this study
| PCR primer | Sequence (5′–3′) |
|---|---|
| GP5+ | TTTGTTACTGTGGTAGATACTAC |
| GP6+ | GAAAAATAAACTGTAAATCATATTC |
| MY09 | CGTCCMARRGGAWACTGATC |
| MY11 | GCMCAGGGWCATAAYAATGG |
| PC04 | CAACTTCATCCACGTTCACC |
| GH20 | GAAGAGCCAAGGACAGGTAC |
| Real-time PCR primer and TaqMan probe | Sequence (5′–3′) |
| E2-F | AACGAAGTATCCTCTCCTGAAATTATTAG |
| E2-R | CCAAGGCGACGGCTTTG |
| E2-Probe | FAM-CACCCCGCCGCGACCCATA-TAMRA |
| E6-F | GAGAACTGCAATGTTTCAGGACC |
| E6-R | TGTATAGTTGTTTGCAGCTCTGTGC |
| E6-Probe | FAM-CAGGAGCGACCCAGAAAGTTACCACAGTT-TAMRA |
| β-globin-F | TGGGTTTCTGATAGGCACTGACT |
| β-globin-R | AACAGCATCAGGAGTGGACAGAT |
| β-globin-Probe | FAM-TCTACCCTTGGACCCAGAGGTTCTTTGAGT-TAMRA |
| β-actin-F | GCGAGAAGATGACCCAGATC |
| β-actin-R | CCAGTGGTACGGCCAGAGG |
| β-actin-Probe | FAM-CCAGCCATGTACGTTGCTATCCAGGC-TAMRA |
F Forward; R Reverse