Fig. 4.

C-MYC overexpression reduced osteogenic and adipogenic markers upon MSC differentiation. a–c c-MYC-MSC or GFP-MSC were expanded to passage 8, and osteogenic differentiation was induced for 3 weeks (D0–D21). a c-MYC mRNA expression measured by qRT-PCR; n = 2. b Total protein content monitored with Bradford reagent at day 14. c Calcium deposition measured by Alizarin Red S staining and normalized to total protein content; d, e C-MYC-MSC or GFP-MSC were expanded to passage 8, and adipogenic differentiation was induced for 3 weeks. d Top: representative images of MSC stained with Oil Red O; scale bar, 100 μm; bottom: quantitative analysis of Oil Red O content; n = 4; *P < 0.05. b–d MSC were from n = 4 donors with combined cells at passages 4, 6, 8, and 10; *P < 0.05, versus GFP control at similar time point. e PPARG mRNA expression measured by qRT-PCR; n = 3, passage 8; *P < 0.05