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. 2019 Mar 5;16:17. doi: 10.1186/s12986-019-0344-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Effects of PPARγ agonist and antagonist on chemerin level in the serums (a), livers (b) and gastrocnemius (c) of EDM rats. PPARγ antagonist GW9662 had no influence on exercise-induced decrease of chemerin in serum (a) but significant reversed the decreases of chemerin in the livers (b) and gastrocnemius (c) of EDM rats at mRNA (upper) and protein (middle) levels. Pioglitazone further strengthened the down-regulations of chemerin in the serums (a), livers (b) and gastrocnemius (c) of EDM rats. The blots of chemerin were quantified by Tanon software and normalized against β-actin or GAPDH, then the normalized numbers were compared between different groups (bottom). DM: diabetes mellitus; EDM: exercised DM; EDP: EDM + pioglitazone; EDG:EDM + GW9662. ^*P<0.05, ^**P<0.01 vs DM. ^#P<0.05, ^##P<0.01 vs EDM