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. Author manuscript; available in PMC: 2019 Mar 6.
Published in final edited form as: Nature. 2018 Sep 5;561(7723):331–337. doi: 10.1038/s41586-018-0499-y

ED-Figure 7 │. Analysis of T-cell infiltrates in vital organs and normal brain after infusion of HS T-cells.

ED-Figure 7 │

(A) CD3 immunohistochemistry (IHC) staining of normal vital tissues from animals receiving HS T-cells vs. NT control. n=3 mice/group. Scale bar=40μm (B) IHC showing HS T-cell infiltrate in micro-dissected GBM xenograft. Scoring of CD3 positive DAB signal was analyzed using IHC-Profiler® plugin in ImageJ®. Respective image analysis output and the score assigned using IHC-Profiler is also shown for each image. Total percentage of CD3+ DAB signal was more 66% in all mice brain with HS T-cells (score from 3 to 4) while percentage in control mice were less than 20% (score were 0–1). Scale bar=50μm. n=3 mice/group