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. Author manuscript; available in PMC: 2019 Mar 6.
Published in final edited form as: Nature. 2018 Sep 5;561(7723):331–337. doi: 10.1038/s41586-018-0499-y

ED-Figure 1 │. Analysis of CAM expression in primary brain tumors.

ED-Figure 1 │

(A) High-throughput IFC analysis routine of the endothelial adhesion molecules; ICAM1, VCAM1, and ALCAM in primary 93 GBM, 25 MB and 5 normal brain. MATLAB segmentation and masking analysis algorithm of the co-immunofluorescence (co-IFC) of ICAM1 or VCAM (acquired on 647 channel), CD31 (acquired on 594 channel) and ALCAM (acquired on 488 channel), DAPI (acquired on blue/cyan channel). (B) Isolation and characterization of pTEC. Flow-cytometry sorting gating strategy of pTEC from freshly excised glioblastoma (GBM; n=5) based on CD31 positivity. GBM EC isolated also expressed the endothelial markers VE-cadherin, von Willebrand Factor (vWF) and ALCAM. Isotype shown in lighter grey and test shown in darker grey in individual histograms. n=5 surgical samples each interrogated at least twice. 100,000 or more events were acquired per condition.