. Author manuscript; available in PMC: 2019 Mar 6.

Published in final edited form as: ACS Chem Biol. 2019 Feb 1;14(2):186–191. doi: 10.1021/acschembio.8b01079

Table 1:

EC₅₀ and IC₅₀ values and efficacy data for AHL analogs in the E. coli and P. aeruginosa RhlR reporter strains.^a Data for control compounds shaded in grey.

	E. coli			P. aeruginosa
Compound	EC₅₀ (μM)	95% CI (μM)^b	Max. RhlR Activation (%)	EC₅₀ (μM)	95% CI (μM)^b	Max. RhlR Activation (%)
34	5.94	4.19 – 8.41	93	7.35	5.26 – 10.3	96
35	1.72	1.34 – 2.21	110	1.65	1.24 – 2.21	90
36	7.58	5.80 – 9.90	100	11.24	7.41 – 17.1	96
37	0.463	0.336 – 0.640	93	2.58	1.86 – 3.56	91
BHL	8.95	5.86 – 13.7	100	8.08	6.09 – 10.7	100
7	1.02	0.67 – 1.55	110	1.42	1.08 – 1.86	94
17	1.78	1.37 – 2.31	100	1.41	1.14 – 1.74	96
S4	1.58	1.32 – 1.90	100	1.22	1.03 – 1.45	110
	IC₅₀ (μM)	95% CI (μM)^b	Max. RhlR Inhibition (%)	IC₅₀ (μM)	95% CI (μM)^b	Max. RhlR Inhibition (%)
38	26.7	10.1 – 71.0	32	–	–	–
41	>100	–	56	–	–	–
42	19.6	14.3 – 26.9	81	31.4	19.6 – 50.4	85
E22	17.3	12.1 – 24.6	74	23.9	16.6 – 31.6	96

See Methods for assay details. For full dose response curves, see Figures S1–S4.

CI = confidence interval.