Fig. 2.

VPO1 expression is upregulated during cardiac fibrosis after myocardial infarction (MI). (A) Representative images of masson trichrome staining and immunofluorescence staining of MPO (red), VPO1(red), α-SMA (green) and DAPI (blue) in the infarct zone of mouse hearts in sham group and 7 days, 14 days, 28days after MI. Scale bar, 50 μm. (B) Levels of VPO1, MPO, α-SMA and Collagen I in the sham and MI mouse hearts as determined by western blot and quantified by Image Lab software. n = 6. (C) Immunochemical staining of Ki67 (a proliferation marker) and 3-Cl Tyr in sham and post MI at 7 days, 14 days, 28 days. Quantification of Ki67 positive cells and 3-Cl Tyr staining level in sham and subjected MI mouse. n = 6. Scale bar: 50 μm (upper), 20 μm (bottom). Data are presented as mean ± SEM. In B, Student's t-test in comparison with Sham group. *P < 0.05 vs Sham, **P < 0.01 vs Sham, NS: no significance vs Sham. In C, One-way ANOVA test was used. *P < 0.05, **P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)