Fig. 4.

Evaluation of the protective effects of compound 51164 in conditions of amyloid synaptotoxicity. (A) Representative confocal images of day 14 of in vitro cultivation hippocampal neurons transfected with tdTomato plasmid. Images for control (CTRL) cultures and cultures exposed to oligomeric Aβ42 (Aβ) are shown as indicated. HPF was added at the concentration of 30 nM, compound 51164 was tested at concentrations of 1, 10, 30, and 100 nM as indicated. Scale bar, 10 µm. (B) The average percentages of mushroom spines (%MS) in each experimental condition are present as mean ± S.D. (n = 22–32 neurons for each group). Untreated hippocampal cultures (CTRL) are shown as filled bars. Open bars correspond to hippocampal cultures that were treated with oligomeric Aβ42. *P < 0.05; ***P < 0.0005 by two-way ANOVA with Dunn-Sidak post hoc test.