Fig. 5.

Compound 51164 acts as a positive modulator of TRPC6 channels. (A) Time course of Fura-2 fluorescence Ca²⁺ signals (340/380 ratio) is shown for HEK293 cells transfected with EGFP + TRPC6 plasmids. Traces from individual cells are shown as thin gray lines, and average traces are shown as thick black lines. Cells were incubated in aCSF medium containing 2 mM Ca²⁺. The time of addition of 10 µM HPF or 30 µM of compound 51164 is indicated by black bars above the Fura-2 traces. (B) Average amplitude of Ca²⁺ influx peak is shown as the change in 340/380 Fura-2 ratio signals for the cells exposed to HPF or compound 51164. The results are presented as mean ± S.D. (n = 40 cells). ***P < 0.0001 by two-way ANOVA with Tukey’s post hoc test. (C) Time course of Fura-2 fluorescence Ca²⁺ signals (340/380 ratio) is shown for HEK293 cells transfected with EGFP (GFP) or EGFP + TRPC6 (TRPC6) plasmids as indicated. Traces from individual cells are shown as thin gray lines, and average traces are shown as thick black lines. Cells were moved to modified aCSF medium containing 0.1 mM Ca²⁺ for 2 minutes, and then returned to the medium containing 2 mM Ca²⁺ with the addition of 50 µM of OAG. The time of addition of 30 µM of compound 51164 is indicated by black bars above the Fura-2 traces. (D) Average amplitude of Ca²⁺ influx peak is shown as the change in 340/380 Fura-2 ratio signals for each group of cells tested in experiments shown in (C). The results are presented as mean ± S.D. (n = 40 cells). ***P < 0.0001 by two-way ANOVA with Tukey’s post hoc test.