Figure 5.

Notch activation in Defa4^Cre-expressing cells leads to loss of secretory cell differentiation and increased expression of the stem cell marker Olfm4. Histologic and immunofluorescent staining of the small intestine from Defa4^Cre;Rosa26^EYFP and Defa4^Cre;Rosa26^{NICD-ires-nGFP} mice. (A) PAS/Alcian blue staining. Dashed line denotes wild-type crypt in jejunum of Defa4^Cre;Rosa26^{NICD-ires-nGFP} intestine. (B) Immunofluorescent staining of GFP or NICD (nGFP+) with cell type–specific markers, lysozyme, MUC2, chromogranin A, and villin. (C) Immunofluorescent staining of GFP or NICD (nGFP+) with the stem cell marker Olfm4. Scale bars: 50 μm.