Figure 6.

Inducible Notch activation in adult Defa4^Cre-expressing cells allows for dedifferentiation and lineage tracing. (A) Schematic of inducible Notch activation in adult Defa4^Cre-expressing cells. In the presence of doxycycline, reverse tetracycline transactivator will bind the tetracycline response element (TRE) in TetO^NICD allele and activate NICD expression in Defa4^Cre-expressing cells. Defa4^Cre;Rosa26^{rtTA-ires-EGFP};TetO^NICD mice (N = 5) received 2 mg/mL doxycycline in water for 2 weeks. (B) Immunofluorescent staining with GFP antibody. Top: No doxycycline (N = 4). Bottom: Two weeks on doxycycline (N = 5). Scale bars: 20 μm.