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. 2019 Mar 1;12:1765–1779. doi: 10.2147/OTT.S196142

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© 2019 Hou et al. This work is published and licensed by Dove Medical Press Limited

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TAZ deletion induces apoptosis in liver cancer cells in vitro.

Notes: (A, B) Two independent TAZ shRNAs were transfected into HepG2 liver cancer cells. Western blotting was used to observe the knockdown efficiency. (C) The MTT assay was used for cell viability detection. Two independent TAZ shRNAs were transfected into HepG2 liver cancer cells. (D) LDH release assay for cell death. (E, F) TUNEL staining for apoptotic cells. Green dots were recorded, and the ratio of TUNEL-positive cells was evaluated to reflect cell apoptosis. (G) Caspase-3 activity was measured via ELISA. Two independent shRNAs were transfected into HepG2 liver cancer cells. (H–J) Western blotting was performed to analyze the expression of pro-apoptotic proteins, such as cleaved caspase-3 and cleaved PARP. *P<0.05 vs sh-ctrl.

Abbreviations: Pro. caspase-3, pro-apoptotic caspase-3; Cle. caspase-3, cleaved caspase-3; LDH, lactate dehydrogenase; sh-ctrl, control shRNA; TAZ, transcriptional co-activator with PDZ-binding motif.