Figure 2.

TAZ deletion impairs HepG2 liver cancer cell proliferation and migration.

Notes: (A, B) EdU assays were performed to verify the functional role of TAZ in HepG2 cell growth. The number of EdU-positive cells was recorded. (C–E) To assess the cellular response to TAZ deletion, protein was collected, and Western blotting was used to analyze the expression of Cyclin D1 and CDK4. (F, G) Transwell assays were used to observe HepG2 cell migration. Z-VAD-FMK was used to exclude the influence of apoptosis on cell migration. (H, I) RNA was isolated from HepG2 cells, and qPCR was conducted to analyze the expression of ROCK1 and Rac1, which are key factors involved in the cancer cell migratory response. *P<0.05 vs sh-ctrl (control shRNA).

Abbreviations: sh-ctrl, control shRNA; TAZ, transcriptional co-activator with PDZ-binding motif.