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. 2019 Mar 1;12:1765–1779. doi: 10.2147/OTT.S196142

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© 2019 Hou et al. This work is published and licensed by Dove Medical Press Limited

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TAZ deletion triggers mitochondrial fission in a manner dependent on MIEF1.

Notes: (A–G) Western blotting was applied to evaluate the expression of pro-fission proteins, including Drp1, MIEF1 and Fis1. Pro-fusion factors, such as Mfn1, Mfn2 and Opa1, were also evaluated via Western blotting. (H, I) Immunofluorescence assay for mitochondria using a mitochondrial-specific antibody Tom-20. The average length of the mitochondria was measured, and this parameter was used to quantify mitochondrial fission. (J, K) The expression of MIEF1 was also evaluated via Western blotting. shRNA against MIEF1 was transfected into HepG2 cells, and the expression of MIEF1 was subsequently measured. *P<0.05 vs sh-ctrl, ^#P<0.05 vs sh-TAZ.

Abbreviations: MIEF1, mitochondrial elongation factor 1; sh-ctrl, control shRNA; TAZ, transcriptional co-activator with PDZ-binding motif.

TAZ deletion triggers mitochondrial fission in a manner dependent on MIEF1.

Notes: (A–G) Western blotting was applied to evaluate the expression of pro-fission proteins, including Drp1, MIEF1 and Fis1. Pro-fusion factors, such as Mfn1, Mfn2 and Opa1, were also evaluated via Western blotting. (H, I) Immunofluorescence assay for mitochondria using a mitochondrial-specific antibody Tom-20. The average length of the mitochondria was measured, and this parameter was used to quantify mitochondrial fission. (J, K) The expression of MIEF1 was also evaluated via Western blotting. shRNA against MIEF1 was transfected into HepG2 cells, and the expression of MIEF1 was subsequently measured. *P<0.05 vs sh-ctrl, ^#P<0.05 vs sh-TAZ.

Abbreviations: MIEF1, mitochondrial elongation factor 1; sh-ctrl, control shRNA; TAZ, transcriptional co-activator with PDZ-binding motif.