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. 2019 Mar 1;12:1765–1779. doi: 10.2147/OTT.S196142

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© 2019 Hou et al. This work is published and licensed by Dove Medical Press Limited

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MIEF1-related mitochondrial fission modulates mitochondrial stress.

Notes: (A, B) Mitochondrial ROS production was analyzed using flow cytometry. The relative ROS content was recorded as a ratio to that of the control group. shRNAs against TAZ and MIEF1 were transfected into HepG2 cells. (C–E) The concentrations of cellular antioxidants, such as GSH, GPX, and SOD, were measured using ELISA. (F, G) Immunofluorescence assay for cyt-c liberation. Relative nuclear cyt-c expression was recorded as a ratio to that of the control group. (H–L) Western blotting analysis was used to analyze the alterations of mitochondrial apoptotic proteins, such as caspase-9, Bax, Bcl-2, and c-IAP1. shRNAs against TAZ and MIEF1 were transfected into HepG2 cells. *P<0.05 vs sh-ctrl, ^#P<0.05 vs sh-TAZ.

Abbreviations: MIEF1, mitochondrial elongation factor 1; ROS, reactive oxygen species; sh-ctrl, control shRNA; TAZ, transcriptional co-activator with PDZ-binding motif.

MIEF1-related mitochondrial fission modulates mitochondrial stress.

Notes: (A, B) Mitochondrial ROS production was analyzed using flow cytometry. The relative ROS content was recorded as a ratio to that of the control group. shRNAs against TAZ and MIEF1 were transfected into HepG2 cells. (C–E) The concentrations of cellular antioxidants, such as GSH, GPX, and SOD, were measured using ELISA. (F, G) Immunofluorescence assay for cyt-c liberation. Relative nuclear cyt-c expression was recorded as a ratio to that of the control group. (H–L) Western blotting analysis was used to analyze the alterations of mitochondrial apoptotic proteins, such as caspase-9, Bax, Bcl-2, and c-IAP1. shRNAs against TAZ and MIEF1 were transfected into HepG2 cells. *P<0.05 vs sh-ctrl, ^#P<0.05 vs sh-TAZ.

Abbreviations: MIEF1, mitochondrial elongation factor 1; ROS, reactive oxygen species; sh-ctrl, control shRNA; TAZ, transcriptional co-activator with PDZ-binding motif.