Figure S2. Sucrose gradient analysis of ribosomes after abrogating assembly of the 40S or 60S ribosomal subunit.

(A–P) Sucrose gradient analysis of ribosomes before and after the cessation of the synthesis of the indicated 60S (A–D and M–N) or 40S (E–L and O–P) r-proteins. Cells were grown in YEP-galactose medium and shifted to YPD medium for the indicated time before harvest. In panels M–O, the spectrophotometer was started a little earlier during gradient tapping to ensure capturing the full peak of A²⁶⁰ material at the top of the gradient.