Figure S6. Titration of antisera against the amount of extract loaded on Western blots.

Slots of a 15% SDS polyacrylamide gel were loaded with increasing numbers of A²⁸⁰ units of whole cell lysate from BY4741 grown in YEP-galactose medium. After electrophoresis and blotting, the membrane was probed with a cocktail of antisera against uS4, uL4, uL5, or uL18. The intensity of the bands was determined using ImageJ, and the relative band intensities are plotted against the number of A²⁸⁰ units loaded in each slot.