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. 2019 Feb 18;11(4):1226–1239. doi: 10.18632/aging.101831

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Copyright © 2019 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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graphic file with name aging-11-101831-g007.jpg — Figure 7. Methane restrained pyroptosis in vitro. (A) Representative propidium iodide (PI) staining of RAW 264.7 cells (Scale bars: 25μm). (B) The percentage of PI positive cells. (C) Representative immunoblots of p65, p-p65, pro-caspase1, pro-IL-1β and NLRP3/Caspase-1/IL-1βfrom RAW264.7 cells. (D) Relative densities of p-P65 and P65, (E) NLRP3, (F) caspase-1 and pro-Caspase-1, and (G) IL-1β and pro-IL-1β were calculated. (H) Representative immunofluorescence staining of IL-1β (Scale bars: 25μm). (I) The percentage of IL-1β positive cells was calculated. (n = 3. Data are shown as the mean ±SD. ^#P < 0.05 versus NM (normal medium) group;^$p < 0.05 versus MRM (methane-rich medium) group; ^&P < 0.05 versus. NM+L+A (normal medium+ lipopolysaccharide+ adenosine-triphosphate) group).