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. Author manuscript; available in PMC: 2019 Mar 6.
Published in final edited form as: Methods Cell Biol. 2018;144:33–74. doi: 10.1016/bs.mcb.2018.03.040

Fig. 4.

Fig. 4

A. Western-blot analysis of U2OS cell lysates stably expressing H2B-mRFP and GFP-α-Tub (GEEY, GEE* or GE*) in asynchronous and mitotic populations (cells treated with STLC for 14h). B. Western-blot analysis of U2OS cell lysates stably expressing H2B-mRFP and GFP-α-Tub (GEEY, GEE* or GE*) in control (sgcontrol) and TTL KO cells (sgTTL). C. Deconvolved immunofluorescence showing the cellular distribution of ?2-tubulin in U2OS cells stably expressing H2B-mRFP and GFP-GEE* in the presence or absence of TTL. Scale bar, 5 μm. D. Western-blot analysis of U2OS cells lysates stably expressing H2B-mRFP and NOTAG-α-Tub (GEEY, GEE* or GE*), in control and TTLKO (sgTTL) cells.