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. Author manuscript; available in PMC: 2019 Mar 6.
Published in final edited form as: Methods Cell Biol. 2018;144:33–74. doi: 10.1016/bs.mcb.2018.03.040

Fig. 6.

Fig. 6

A. Immunofluorescence of U2OS cells before and after cold-induced MT destabilization. B. Sum projection of the tubulin channel showing the mitotic spindle circumscribed by two ROIs (small area and big area) used to quantify the fluorescence intensity at the spindle region.