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. Author manuscript; available in PMC: 2019 Mar 6.
Published in final edited form as: Methods Cell Biol. 2018;144:33–74. doi: 10.1016/bs.mcb.2018.03.040

Fig. 8.

Fig. 8

A. Western blot analysis of mitotic HeLa cells lysates after treatment with siRNA for TTL. B. Schematic diagram of the protocol used for the purification of MAPs and motors from mitotic extracts. Protocol adapted from (Sloboda, 2015). C. Silver staining analysis of fractions obtained following protocol B.