. Author manuscript; available in PMC: 2019 Mar 6.

Published in final edited form as: Methods Cell Biol. 2018;144:33–74. doi: 10.1016/bs.mcb.2018.03.040

Table 8. Oligonucleotides sequences.

Number	Name	Primer Sequence (5' to 3')
1	XbaIF	`GCTCTAGAATGGTGAGCAAGGGCGAGG`
2	KpnIR	`GGGGTACCTTAGTATTCCTCTCCTTCTTCCTC`
3	Y450*F	`CGCGGATCCTTATTATTCCTCTCCTTCTTCCTCA`
4	Y450*R	`TGAGGAAGAAGGAGAGGAATAATAAGGATCCGCG`
5	pEGFPC1F	`GATCACTCTCGGCATGGACG`
6	TubSEQ	`CACTGGCTTCAAGGTTGGCATC`
7	E449Y450F	`TGAGGAAGAAGGAGAGTAATAATAAGGATCCGCGGCCG`
8	E449Y450F	`CGGCCGCGGATCCTTATTATTACTCTCCTTCTTCCTCA`
9	CMV-F	`CGCAAATGGGCGGTAGGCGTG`
10	Antis_del	`GTGGATGGAGATGCACTCACGCATTCTAGAGTCGGTGTCTTC`
11	Sens_del	`GAAGACACCGACTCTAGAATGCGTGAGTGCATCTCCATCCAC`
12	EcoRV_R	`GCTTTCTCAGCAGAGATGAC`
13	hU6F	`GAGGGCCTATTTCCCATGATT`