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Evaluation of the inhibition of SIRT1 and SIRT2 by different inhibitors in cells. (A) Detection of acetyl p53 (Ac-p53) levels in MCF-7 cells after 6-hour treatment with indicated inhibitors and 200 nM of TSA, an HDAC inhibitor. (B) Detection of acetyl α-tubulin (Ac-α-tubulin) levels by immunofluorescence in MCF-7 cells after 6-hour treatment with indicated inhibitors.
