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. Author manuscript; available in PMC: 2019 Jul 7.
Published in final edited form as: Nat Neurosci. 2019 Jan 7;22(2):243–255. doi: 10.1038/s41593-018-0295-x

Figure 6: Transgenic overexpression of proneural transcription factors in iPSCs (iPSC-iNs) circumvents proliferative NSC-like stages.

Figure 6:

a, Schematic of lentiviral system for inducible overexpression of Ngn2-2A-eGFP (top). Direct conversion of iPSCs into induced neurons (iNs) by forced expression of an inducible Ngn2 transgene may bypass early NSC-like stages (bottom). b, Representative images of patient-derived iPSC-iNs at different stages in the course of neuronal differentiation. Scale bar 200 μm. Experiments were repeated in all 13 patient lines at 4 different time points. c, Immunocytochemical characterization of iPSC-iNs after two weeks of conversion. Scale bar represents 25 μm. Immunohistochemistry was performed in all 13 patient lines at least once with similar results d, BrdU-labeling experiment with unselected iPSC-iNs during conversion (arrowheads highlight GFP-expressing iPSC-iNs). Scale bar 50 μm. Values represent mean ± s.d.; n=4 independent control cell lines. e, Representative reconstructions of patient-derived iPSC-iNs at the stages indicated in b. Scale bar 100 μm. Experiments were repeated in all 13 patient lines at 4 different time points. Also see Extended Data Figures 8 and 9.