Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2020 Mar 15.

Published in final edited form as: Toxicol Appl Pharmacol. 2019 Feb 13;367:71–81. doi: 10.1016/j.taap.2019.02.006

Figure 1. — (A) THP-1 derived macrophage mRNA expression for TNF, IL-1β, IL-6, IL-8, CCL2 (MCP1), CCL3 (MIP-1α), CCL4 (MIP-1β) were assessed after 24 h treatment of PCB 126, PCB126 + LPS (10 ng/mL)/IFN-γ (5 ng/mL) and DMSO. (B) Protein levels for cytokines TNF, IL-1β, IL-8, CCL2, CCL3, CCL4, IL-1RA, IL-1, IP-10, and IFN-ɣ were measured utilizing Milliplex Map Human Cytokine/Chemokine Magnetic Bead Panel from cell culture medium after 24 h exposure of PCB 126. IL-6, IL-17A concentrations were below the lower limit of detection (<3.2 pg/mL) (data not shown). Values are mean ± SEM (n=3). Different letters indicate significant difference (P ≤ 0.05) between treatment groups. Tukey’s multiple comparisons test was used.

Figure 1. — (A) THP-1 derived macrophage mRNA expression for TNF, IL-1β, IL-6, IL-8, CCL2 (MCP1), CCL3 (MIP-1α), CCL4 (MIP-1β) were assessed after 24 h treatment of PCB 126, PCB126 + LPS (10 ng/mL)/IFN-γ (5 ng/mL) and DMSO. (B) Protein levels for cytokines TNF, IL-1β, IL-8, CCL2, CCL3, CCL4, IL-1RA, IL-1, IP-10, and IFN-ɣ were measured utilizing Milliplex Map Human Cytokine/Chemokine Magnetic Bead Panel from cell culture medium after 24 h exposure of PCB 126. IL-6, IL-17A concentrations were below the lower limit of detection (<3.2 pg/mL) (data not shown). Values are mean ± SEM (n=3). Different letters indicate significant difference (P ≤ 0.05) between treatment groups. Tukey’s multiple comparisons test was used.