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. Author manuscript; available in PMC: 2020 Mar 15.

Published in final edited form as: Toxicol Appl Pharmacol. 2019 Feb 13;367:71–81. doi: 10.1016/j.taap.2019.02.006

Figure 4. — THP-1 derived macrophages were pretreated with the AhR antagonist CH-223191 (5 M) or the NF B antagonist BMS-345541 (5 M) for 2 h before PCB 126 (500 nM, 24 h) exposure. (A) mRNA expression for TNF, IL-1β, CYP1A1 were assessed. (B) Protein levels for cytokines TNF, IL-1β, IL-8, CCL2, CCL3, CCL4, IL-1RA, IL-1, IP-10, and IFN-ɣ were measured utilizing Milliplex Map Human Cytokine/Chemokine Magnetic Bead Panel from cell culture medium. IL-6, IL-17A concentrations were below the lower limit of detection (<3.2 pg/mL) (data not shown). Values are mean ± SEM (n=3). * P ≤ 0.05. Tukey’s multiple comparisons test was used.

Figure 4. — THP-1 derived macrophages were pretreated with the AhR antagonist CH-223191 (5 M) or the NF B antagonist BMS-345541 (5 M) for 2 h before PCB 126 (500 nM, 24 h) exposure. (A) mRNA expression for TNF, IL-1β, CYP1A1 were assessed. (B) Protein levels for cytokines TNF, IL-1β, IL-8, CCL2, CCL3, CCL4, IL-1RA, IL-1, IP-10, and IFN-ɣ were measured utilizing Milliplex Map Human Cytokine/Chemokine Magnetic Bead Panel from cell culture medium. IL-6, IL-17A concentrations were below the lower limit of detection (<3.2 pg/mL) (data not shown). Values are mean ± SEM (n=3). * P ≤ 0.05. Tukey’s multiple comparisons test was used.