Table 1. Injection sites.

Coordinate ID	1	2	3	4	5	6	7	8	9	10
X:	0.85	0.85	0.85	0.85	0.85	0.85	0.85	0.85	0.65	0.65
Y:	0.85	0.85	0.85	1.15	1.15	1.15	1.45	1.45	0.85	0.85
Z:	0.7	1.1	1.4	0.7	1.1	1.4	0.7	1.1	1.1	1.4
n Injections (2.3nl)	66	33	33	66	33	33	66	33	66	33
Solution (sham)	PBS	PBS	PBS	PBS	PBS	PBS	PBS	PBS	PBS	PBS
Solution (S)	NMDA	PBS	PBS	PBS	PBS	PBS	PBS	PBS	PBS	PBS
Solution (M)	NMDA	PBS	PBS	PBS	PBS	PBS	NMDA	PBS	PBS	PBS
Solution (L)	PBS	PBS	NMDA	NMDA	NMDA	NMDA	NMDA	NMDA	PBS	PBS
Solution (XL)	NMDA	NMDA	NMDA	NMDA	NMDA	NMDA	NMDA	NMDA	PBS	PBS
Solution (XXL)	NMDA	NMDA	NMDA	NMDA	NMDA	NMDA	NMDA	NMDA	NMDA	NMDA

Table shows injection sites for each lesion group used in the experiment. For each coordinate (1–10) the x/y/z positions of injections are shown. X position refers to mm away from bregma in the rostro-caudal axis. Y position refers to mm away from bregma in the medio-lateral axis. Z position refers to depth from the surface of the brain. For each injection site, a number of 2.3nl injections were made, given by the n injections row. For each injection site, the solution injected is shown depending on the desired lesion extent (sham, S, M, L, XL, XXL). PBS was at 1% dilution. NMDA solution was 10mg/ml dissolved in 1% PBS.

After injection completion, the craniotomy was resealed using silicone elastomer (KwikCast, World Precision Instruments, FL USA) and the skin incision was sutured closed (Silkam 7/0, Braun, Tuttlingen Germany) and cleaned with 1% clorhexidine scrub. Animals were given meloxicam (Metacam; 2mg/kg) injected sub-cutaneously for post-operative analgesia. Mice were removed from the stereotaxic apparatus and placed in a warm recovery chamber (Thermo Scientific, MA USA) (36°C) until recovery from anaesthesia was observed (righting reflex regained). Following surgery, animals were singly housed for 3 days, and then returned to the AutonoMouse home cage.