Table 1. Top 10 genes of the pre-granulosa cell-specific downregulated genes (cluster-I) and Sertoli cell-specific upregulated genes (cluster-II and III)1).
| Gene Symbol | Definition | 4d | 7d | 10d ave. | 15d | 20d ave. | E12.5 2) XY > XX |
|---|---|---|---|---|---|---|---|
| Cluster-I (downregulated on 10d) | |||||||
| Cpa2 | carboxypeptidase A2, pancreatic | -1.6 | -6.0 | -15.4 | -29.0 | -48.8 | -4.9 |
| Gm20265 | -1.6 | -4.1 | -13.2 | -31.9 | -30.5 | -11.3 | |
| Fam196b | predicted gene, EG574403 | -1.9 | -3.2 | -11.4 | -21.4 | -33.3 | -30.6 |
| Mbnl3 | muscleblind-like 3 (Drosophila) | -1.9 | -3.9 | -6.9 | -8.1 | -15.2 | -13.9 |
| Trim66 | tripartite motif-containing 66 | -1.0 | -2.0 | -6.8 | -9.0 | -8.0 | -5.7 |
| Gabrb1 | gamma-aminobutyric acid (GABA-A) receptor, subunit beta 1 | -1.7 | -3.3 | -6.7 | -9.3 | -10.5 | -16.7 |
| Col22a1 | collagen, type XXII, alpha 1 | -1.4 | -4.7 | -6.1 | -5.9 | -8.3 | -4.6 |
| Ppp1r3g | protein phosphatase 1, regulatory (inhibitor) subunit 3G | -1.3 | -3.5 | -6.0 | -6.2 | -7.8 | -3.8 |
| Igf2 | insulin-like growth factor 2 | -1.6 | -2.0 | -5.7 | -6.4 | -13.6 | -4.5 |
| Drp2 | dystrophin related protein 2 | -1.5 | -2.8 | -5.1 | -7.1 | -8.5 | -24.4 |
| Cluster-II (upregulated on 10d) | |||||||
| Miox | myo-inositol oxygenase | -1.0 | -1.2 | 11.9 | 22.6 | 77.8 | 4.0 |
| Pla2g4c | phospholipase A2, group IVC (cytosolic, calcium-independent) | -1.1 | -1.3 | 8.5 | 23.8 | 24.9 | 3.1 |
| Rerg | RAS-like, estrogen-regulated, growth-inhibitor | 1.5 | 3.7 | 7.3 | 12.6 | 9.9 | 29.2 |
| Fam40b | family with sequence similarity 40, member B | 1.3 | 2.7 | 7.0 | 6.8 | 6.0 | 4.0 |
| Synm | synemin, intermediate filament protein | 1.8 | 2.5 | 6.6 | 10.4 | 7.8 | 6.7 |
| Zar1 | zygote arrest 1 | -1.0 | -1.2 | 6.5 | 12.3 | 13.0 | 5.1 |
| Gpm6b | glycoprotein m6b | 1.8 | 4.4 | 6.1 | 3.4 | 3.7 | 2.8 |
| Esrp1 | RNA binding motif protein 35A | -2.3 | 1.9 | 6.0 | 10.7 | 9.0 | 7.0 |
| Egflam | EGF-like, fibronectin type III and laminin G domains | 1.4 | 2.7 | 5.4 | 6.2 | 4.3 | 2.8 |
| Slc45a3 | solute carrier family 45, member 3 | -1.4 | -1.3 | 5.0 | 17.4 | 24.3 | 2.0 |
| Cluster-III (upregulated on 20d) | |||||||
| Hao2 | hydroxyacid oxidase (glycolate oxidase) 3 | 1.4 | 1.3 | 1.2 | 1.5 | 10.4 | 4.2 |
| Aass | aminoadipate-semialdehyde synthase | 1.5 | -1.1 | 1.4 | 2.4 | 10.2 | 7.6 |
| Crym | crystallin, mu | -1.5 | -1.3 | -1.2 | 1.0 | 6.5 | 3.8 |
| Adhfe1 | alcohol dehydrogenase, iron containing, 1 | 1.2 | -1.0 | 1.0 | 1.2 | 5.7 | 4.8 |
| Adh1 | alcohol dehydrogenase 1 (class I) | 1.4 | 1.5 | 1.4 | 1.7 | 4.8 | 3.6 |
| Nceh1 | arylacetamide deacetylase-like 1 | 1.6 | 1.1 | 1.5 | 2.9 | 4.6 | 2.5 |
| Gjb2 | gap junction protein, beta 2 | -1.1 | -1.1 | -1.0 | 1.1 | 4.3 | 28.9 |
| Cdo1 | cysteine dioxygenase 1, cytosolic | -1.3 | -1.2 | 1.3 | 2.5 | 4.2 | 3.2 |
| Cage1 | cancer antigen 1 | -1.1 | 1.0 | 1.9 | 3.7 | 3.8 | 3.1 |
| Mfsd2a | major facilitator superfamily domain containing 2 | -1.4 | -1.5 | 1.4 | 3.2 | 3.5 | 4.1 |
1) Fold changes of top 10 genes in cluster-I (pre-granulosa cell-specific genes downregulated on day 10 post-transplantation) and cluster-II and III (Sertoli cell-specific genes upregulated on days 10 and 20 post-transplantation) (also see Fig 4F).
2) Fold changes from a published dataset using Sry-EGFP Tg lines [47].