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. Author manuscript; available in PMC: 2020 Jan 23.
Published in final edited form as: Neurosci Lett. 2018 Nov 1;692:107–114. doi: 10.1016/j.neulet.2018.10.055

Figure 7. D-serine increases the levels of DISC1 agglomerated form in the insoluble pellet fraction of cortical neuronal lysates.

Figure 7

(A) Western blot analysis of DISC1 and SR levels in the fractions of triton-soluble supernatant and insoluble pellet from cortical neuronal cultures treated with vehicle, D-serine (50μM), D-serine (50μM) + AP5 (50μM), D-serine (50μM) + 7-CK (50μM), glycine (50μM) + lithium (100μM) for 24 hrs. Upper panel: DISC1 levels in the fractions of triton-soluble supernatant and insoluble pellet (15 μg each lane); Lower panel: SR levels in the fractions of triton-soluble supernatant and insoluble pellet (15μg each lane). (B) Graphs show quantification analysis of the agglomerated form of DISC1 (upper panel) and SR (lower panel) in the triton-insoluble pellet fraction of cortical neuronal lysates (* P<0.05 vs. control, # P<0.05 vs. D-serine-treated, t-test, n=3 different experiments).