Table 1.

Oligos

Oligonucleotide name	Sequence	Purpose
RNA-8855	5′-UUUUUUUGAGCUAGAGG-3′	Reconstitution scaffold, RNA component
tDNA-10160	5′-/5Phos/CAAGGGACTGGTC TGAATCGGGAATACGGAACGGAA GATCTGAGTTCTCTTCCCCTCTA GCTCAGGACGTACTGACC-3′	Reconstitution scaffold, template DNA component
ntDNA-10161	5′-GTCAGTACGTCCTGAGCTAG AGGGGAAGAGAACTCAGATCTTC CGTTCCGTATTCCCGATTCAGAC CAGTCC-3′	Reconstitution scaffold, nontemplate DNA component
asDNA-12001	5′-AACAGGTCGATCGGAAGTTG GTAACGTCTTCCATACAACCTCC TTACTACATGCAACC-3′	Antisense DNA oligo used to enhance TE at λtR2 and t500
asDNA-12952	5′-TTGTCCAGCTAGCCTTCAAC CATTGCAGAAGGTATGTTGGAGG AATGATGTACGTTGG-3′	Antisense DNA oligo used to enhance TE at thisL
dsDNA-11033	5′-/5Phos/CTTGCCTGACTAG TCTTTCAGGCGATGTGTGCTGGA AGAGATTCAGAGTCTTCCAGTGG −3′	Template-strand dsDNA used to determine EC elongation rates
dsDNA-11034	5′-/5Biosg/CCACTGGAAGAC TCTGAATCTCTTCCAGCACACAT CGCCTGAAAGACTAGTC AGG-3′	Nontemplate-strand dsDNA used to determine EC elongation rates
primer-10401	5′-GGTGGTCCTTGGCTCTGGCG GTGATAATGGTT-3′	Forward primer for amplification of λtR2 and t500
primer-10402	5′-/5Biosg/CGCTTCTGCGTT CTGATTTA-3′	Reverse primer to use with 10401
primer-10517	5′-GTTGTTCCTTGGAAACACCA CCATCATCACCA-3′	Forward primer for amplification of thisL
primer-10518	5′-/5Biosg/CGGCCTGATTTC TGAATAGC-3′	Reverse primer to use with 10518
primer-12285	5′-CCCGAAAAGTGCCACCTGAC G-3′	Forward primer for amplification of PlacUV5
primer-12286	5′-CAGTTCCCTACTCTCGCATG G-3′	Reverse primer to use with 12285