Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | Mouse/Ubiad1Ki/Ki (UBIAD1 (N100S)):C57BL/6J | This paper | N/A | Heterozygous knockin mice harboring mutations in the endogenous Ubiad1 gene that change Asparagine-100 to a Serine residue |
| Genetic reagent (M. musculus) |
Mouse/Ubiad1Ki/Ki
(UBIAD1 (N100S)): C57BL/6 |
This paper | Homozygous knockin mice harboring mutations in the endogenous Ubiad1 gene that change Asparagine-100 to a Serine residue |
|
| Genetic reagent (M. musculus) |
Mouse/HmgcrKi/Ki
(HMGCR K89R/ K248R):C57BL/6 |
PMID: 27129778 |
N/A | |
| Cell line | Mouse Embryonic Fibroblast- Ubiad1WT/WT |
This paper | N/A | Mouse embryonic fibroblasts from wild type C57BL/ 6 mice |
| Cell line | Mouse Embryonic Fibroblast- Ubiad1Ki/Ki |
This paper | N/A | Mouse embryonic fibroblasts from Ubiad1Ki/Ki C57BL/6 mice |
| Cell line | Mouse Embryonic Fibroblast- HmgcrWT/WT |
This paper | N/A | Mouse embryonic fibroblasts from wild type C57BL/ 6 mice |
| Cell line | Mouse Embryonic Fibroblast- Hmgcr Ki/Ki |
This paper | N/A | Mouse embryonic fibroblasts from HmgcrKi/Ki C57BL/6 mice |
| Antibody | Rabbit monoclonal anti-SREBP-1 |
PMID: 28244871 |
IgG-20B12 | |
| Antibody | Rabbit monoclonal anti-SREBP-2 |
PMID: 25896350 |
IgG-22D5 | |
| Antibody | Rabbit polyclonal anti-UBIAD1 |
This paper | IgG-205 | Rabbit polyclonal antibody raised against amino acids 2–21 of mouse UBIAD1; used at 1–5 µg/ml for immunoblots |
| Antibody | Rabbit polyclonal anti- HMGCR |
PMID: 27129778 |
IgG-839c | used at 1–5 µg/ml for immunoblots |
| Antibody | Mouse monoclonal anti- HMGCR |
PMID: 22143767 |
IgG-A9 | used at 1–5 µg/ml for immunoblots |
| Antibody | Rabbit polyclonal anti- Insig-1 |
PMID: 27129778 |
anti-Insig-1 | used at 1:1000 dilution for immunoblots |
| Antibody | Rabbit polyclonal anti-Insig-2 | This paper | IgG-492 | Rabbit polyclonal antibody raised against a C-terminal peptide (CKVIPEKSHQE) of hamster Insig-2; used at 5 µg/ml for immunoblots |
| Antibody | Rabbit polyclonal anti-UBXD8 | PMID: 27129778 | IgG-819 | used at 1–5 µg/ml for immunoblots |
| Antibody | Rabbit polyclonal anti-Calnexin | Novus Biologicals | Cat#NB100-1965; RRID:AB_10002123 | used at 1–5 µg/ml for immunoblots |
| Antibody | Rabbit polyclonal anti-GM130 | Abcam | Cat#ab30637; RRID:AB_732675 | used at 1–5 µg/ml for immunoblots |
| Antibody | Rabbit polyclonal anti-LSD-1 | Cell Signaling Technology | Cat#2139; RRID:AB_2070135 | used at 1–5 µg/ml for immunoblots |
| Antibody | Mouse monoclonal anti-ubiquitin (IgG-P4D1) | Santa Cruz | Cat#SC8017;RRID:AB_628423 | used at 1–5 µg/ml for immunoblots |
| Recombinant DNA reagent | ||||
| Sequence-based reagent |
Ubiad1Ki/Ki genotyping primers: Forward, GGAACACTTGGCTCTCATCT; Reverse, GGGAGCAGTGTTCATAATCC |
This paper | N/A | Genotyping was determined by PCR analysis of genomic DNA prepared from tails of mice. |
| Sequence-based reagent |
HmgcrKi/Ki genotyping primers: K89R- Forward, GTCCATGAACATGTTCACCG; Reverse, CAGCACGTCCTATTGGCAGA K248R – Forward, TCGGTGATGTTCCAGTCTTC; Reverse, GGTGGCAAACACCTTGTATC |
PMID: 27129778 | N/A | |
| Sequence-based reagent (qRT-PCR) | UBIAD1 Forward, GACAGAACTTTGGTGGACAGAATTC; Reverse, CAGCCCAAGGTGTAGAGGAAGA |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | SREBP-1a Forward, GGCCGAGATGTGCGAACT; Reverse, TTGTTGATGAGCTGGAGCATGT |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | SREBP-1c Forward, GGAGCCATGGATTGCACATT; Reverse, GGCCCGGGAAGTCACTGT |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | SREBP-2 Forward, GCGTTCTGGAGACCATGGA; Reverse, ACAAAGTTGCTCTGAAAACAAATCA |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | HMGCR Forward, CTTGTGGAATGCCTTGTGATTG; Reverse, AGCCGAAGCAGCACATGAT |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | Insig-1 Forward, TCACAGTGACTGAGCTTCAGCA;
Reverse, TCATCTTCATCACACCCAGGAC |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | Insig-2a Forward, CCCTCAATGAATGTACTGAAGGATT;
Reverse, TGTGAAGTGAAGCAGACCAATGT |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) |
Insig-2b Forward, CCGGGCAGAGCTCAGGAT;
Reverse, GAAGCAGACCAATGTTTCAATGG |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | SCAP Forward, ATTTGCTCACCGTGGAGATGTT;
Reverse, GAAGTCATCCAGGCCACTACTAATG |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | HMGCS Forward, GCCGTGAACTGGGTCGAA;
Reverse, GCATATATAGCAATGTCTCCTGCAA |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | FPPS Forward, ATGGAGATGGGCGAGTTCTTC;
Reverse, CCGACCTTTCCCGTCACA |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | SqS Forward, CCAACTCAATGGGTCTGTTCCT;
Reverse, TGGCTTAGCAAAGTCTTCCAACT |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | LDLR Forward, AGGCTGTGGGCTCCATAGG;
Reverse, TGCGGTCCAGGGTCATCT |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | PCSK9 Forward, CAGGCGGCCAGTGTCTATG;
Reverse, GCTCCTTGATTTTGCATTCCA |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | ACS Forward, GCTGCCGACGGGATCAG;
Reverse, TCCAGACACATTGAGCATGTCAT |
Integrated DNA Technologies | N/A | |
| Sequence-based reagent (qRT-PCR) | ACC1 Forward, TGGACAGACTGATCGCAGAGAAAG;
Reverse, TGGAGAGCCCCACACACA |
Integrated DNA Technologies |
N/A | |
| Sequence- based reagent (qRT-PCR) |
FAS Forward, GCTGCGGAAACTTCAGGAAAT;
Reverse, AGAGACGTGTCACTCCTGGACTT |
Integrated DNA Technologies |
N/A | |
| Sequence- based reagent (qRT-PCR) |
SCD1 Forward, CCGGAGACCCCTTAGATCGA;
Reverse, TAGCCTGTAAAAGATTTCTGCAAACC |
Integrated DNA Technologies |
N/A | |
| Sequence- based reagent (qRT-PCR) |
GPAT Forward, CAACACCATCCCCGACATC;
Reverse, GTGACCTTCGATTATGCGATCA |
Integrated DNA Technologies |
N/A | |
| Sequence- based reagent (qRT-PCR) |
LXRα Forward, TCTGGAGACGTCACGGAGGTA;
Reverse, CCCGGTTGTAACTGAAGTCCTT |
Integrated DNA Technologies |
N/A | |
| Sequence- based reagent (qRT-PCR) |
ABCG5 Forward, TGGATCCAACACCTCTATGCTAAA;
Reverse, GGCAGGTTTTCTCGATGAACTG |
Integrated DNA Technologies |
N/A | |
| Sequence- based reagent (qRT-PCR) |
ABCG8 Forward, TGCCCACCTTCCACATGTC;
Reverse, ATGAAGCCGGCAGTAAGGTAGA |
Integrated DNA Technologies |
N/A | |
| Sequence- based reagent (qRT-PCR) |
GGPS Forward, CGTCTACTTCCTTGGACTGGAAA;
Reverse, AGCTGGCGTGTGAAAAGCTT |
Integrated DNA Technologies |
N/A | |
| Sequence- based reagent (qRT-PCR) |
Cyclophilin Forward, TGGAGAGCACCAAGACAGACA;
Reverse, TGCCGGAGTCGACAATGAT |
Integrated DNA Technologies |
N/A | |
| Commercial assay or kit |
TaqMan Reverse Transcription |
Applied Biosystems |
Cat#N8080234 | |
| Commercial assay or kit |
Power SYBR Green PCR Master Mix |
Applied Biosystems |
Cat#4367659 | |
| Commercial assay or kit |
Cholesterol/ Cholesterol Ester Assay Kit - Quantitation |
Abcam | Cat#ab65359 | |
| Chemical compound, drug |
Cholesterol | Bio-Serv; | Cat#5180; | |
| Chemical compound, drug |
Sigma-Aldrich | Cat#C8667 | ||
| Chemical compound, drug |
Coenzyme Q-10 | Cerilliant | Cat#V-060 | |
| Chemical compound, drug |
Geranylgeraniol | Sigma-Aldrich | Cat#G3278 | |
| Chemical compound, drug |
Geranylgeranyl pyrophosphate |
Cayman Chemical Company |
Cat#63330 | |
| Chemical compound, drug |
Lovastatin | Abblis Chemicals LLC, Houston, TX |
Cat#AB1004848 | |
| Chemical compound, drug |
Menaquinone-4 | Sigma-Aldrich | Cat#809896 | |
| Chemical compound, drug |
Cerilliant | Cat#V-031 | ||
| Chemical compound, drug |
Menaquinone-7 | Cerilliant | Cat#V-044 | |
| Chemical compound, drug |
Phylloquinone (Vitamin K1) |
Cerilliant | Cat#V-030 | |
| Chemical compound, drug |
25-Hydroxycholesterol | Avanti Polar Lipids |
Cat#700019P | |
| Software, algorithm |
Image Studio v5.0 |
LiCor Biosciences |
||
| Software, algorithm |
Image J (Fiji) | NIH |