Fig 4. Visualization of 11P6 in CaMV_11P6-infected A. thaliana GFP1-10 plants.

(A) GFP1-10 plants were mechanically inoculated at different times with CaMV_11P6 and analyzed at the indicated day post inoculation (dpi) for GFP fluorescence with a fluorescence scanner. The figure is a collage from different acquisitions and plants are presented at different magnification scales. Two plants are presented for 28 dpi and 36 dpi to show different infection states. Non-infected and CaMV_wt-inoculated (21 dpi) plants are included as negative controls. (B) 11P6 fluorescence is observed before appearance of visual symptoms. Arabidopsis thaliana GFP1-10 plants were mechanically inoculated with CaMV_11P6 and leaves analyzed at 24 dpi for GFP fluorescence and visual symptoms with a G:Box (upper panel). The leaves were also analyzed for symptoms with a color camera (lower panel). The three images present 11P6 fluorescence in symptomatic and unsymptomatic leaves. The various leaves in the image to the right are from the same plant. Note that the leaves indicated by the yellow arrowheads are not yet infected. (C) The two pictures show negative controls where either CaMV_11P6 was inoculated in Col0 plants or CaMV_wt in GFP1-10 plants as indicated. Images were acquired at 32 dpi with a G:Box or a color camera as described above.