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. 2019 Mar 6;5(3):eaav2448. doi: 10.1126/sciadv.aav2448

Table 1. List of human cell lines used in this study.

CIMA, Centro de Investigación Médica Aplicada, Spain; CRG, Center for Genomic Regulation, Spain; EC, epithelial carcinoma; FBS, fetal bovine serum (Hyclone); IMIM, Institut Hospital del Mar d’Investigacions Mèdiques, Spain; IRB, Research Institute Barcelona, Spain; LC, leukemic cells; NaPyr, sodium pyruvate (Gibco); P/S, penicillin-streptomycin (Gibco); Qx, glutaMAX (Gibco); RPMI 1640 (Gibco); GM-CSF, granulocyte-macrophage colony-stimulating factor; h.i., heat inactivated.

Cell line name Origin Obtained from Media Estimated
doubling time
HEL LC Puri Fortes’ lab (CIMA) 90% RPMI 1640 + 10 FBS + MEM + P/S + Qx + NaPyr 35 hours
NB4 LC Luciano Di Croce’s lab (CRG) 90% RPMI 1640 + 10 FBS + P/S + Qx 35–45 hours
OCI-AML2 LC Puri Fortes’ lab (CIMA) 90% DMEM +10 FBS + MEM + P/S + Qx + NaPyr 30–50 hours
Skno-1 LC DSMZ (Deutsche Sammlung von
Mikroorganismen und Zellkulturen)
90% RPMI 1640 + 10% FBS + GM-CSF 35–50 hours
K562 LC Luciano Di Croce’s lab (CRG) 90% RPMI 1640 + 10 FBS + P/S + Qx 30–40 hours
LoVo EC IMIM repository 90% RPMI 1640 + 10 FBS + MEM + P/S + Qx + NaPyr 48 hours
SW480 EC IMIM repository DMEM + 10% FBS + 10 FBS + P/S + Qx 25–30 hours
HT-29 EC IMIM repository DMEM + 10% FBS + 10 FBS + P/S + Qx 40–60 hours
MCF7 EC CRG repository DMEM + 10% FBS + P/S + insulin 30–72 hours
T47D EC Miguel Beato’s lab (CRG) 90% RPMI 1640 + 10 h.i. FBS + MEM + P/S + Qx + NaPyr 30–40 hours
Fadu EC Salvador Aznar-Benitah’s lab (IRB) MEM + 10%FBS + P/S + PyrNa 30 hours