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. 2019 Jan 15;27(3):531–541. doi: 10.1016/j.ymthe.2019.01.006

Figure 1.

Figure 1

Characterization and Expression Analysis of circRNA.33186

(A) Schematic diagram of circRNA.33186 formed by back-splicing from the mouse Umad1 gene at chromosome 6. (B) Divergent primers detected circular RNAs in complementary DNA (cDNA), but not in genomic DNA (gDNA). (C) Sanger sequencing showed the back-splice junction (arrow) of circRNA.33186. (D) qRT-PCR analysis of circRNA.33186 expression in primary chondrocytes stimulated with 10 ng/mL IL-1β for 0, 12, 24, 48, or 72 h and at 0, 5, 10, 50, or 100 ng/mL for 24 h. (E) Representative pictures of articular cartilage stained by H&E. Scale bar, 100 μm. (F) qRT-PCR analysis of circRNA.33186 expression in DMM-induced OA cartilage tissues (n = 10). *p < 0.01.