Figure 5.

Helios⁻ Treg mice fail to fully inhibit the expansion of effector scurfy cells. Splenocytes from 21d scurfy mice were injected r.o. into 8 wk old B6.SJL RAG^−/− recipients. Helios⁻ or Helios⁺ Treg cells were co-injected where indicated. After 6 weeks, splenocytes were (A) quantified (left panel), gated on scurfy CD45.1⁺TCRβ⁺ cells and CD8⁺ cells (middle panel) or CD4⁺ cells quantified (right panel) (B) Scurfy CD4⁺ cells, scurfy CD8⁺ cells or Treg (CD45.2⁺TCRβ⁺) were analyzed by intracellular staining for IFNγ expression. Data shown as the percent of the gated cells that express IFNγ. (C) Liver and lung tissue were taken at 6 wks, stained for H&E and the degree of infiltration scored. (D) Helios⁻ or Helios⁺ Treg (CD45.2⁺TCRβ⁺) were quantified (left) analyzed for RFP (Foxp3) expression (middle) and the total Foxp3⁺ Treg calculated (right). All data shown as mean ± SD, from two of three independent experiments with n=3 to 5 mice per experiment.