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. Author manuscript; available in PMC: 2020 Apr 2.
Published in final edited form as: Biochem Biophys Res Commun. 2019 Feb 21;511(2):416–421. doi: 10.1016/j.bbrc.2019.02.073

Fig. 2.

Fig. 2.

One-dimensional slices from two-dimensional NOESY spectra of the pseudouridine-modified U2 snRNA – BPS-containing RNA duplex in 100% D2O with 400 ms mixing times at (a) 15 °C and (b) 25 °C show that residues G7 and U6 are dynamic and exhibit large populations of C2′-endo sugar puckers. Each slice is taken through the aromatic proton indicated in bold type at the left of the slice. In (a), conformational dynamics causes broad resonances for G7H3′ and G7H2′. In (b), the 4 Hz scale bar shows the 4–5 Hz H1′-H2′ scalar coupling for G7 and U6 which can be compared to the negligible H1′-H2′ coupling for other residues.