Table 1.

Molecular weight and size of the amplicons produced in the PCR assays invA, RB1 and SED, and amplification efficiencies of the invA and RB1 assays.

	invA qPCR assay	RB1 qPCR assay	SED PCR assay
MWamplicon (Da)	54407a	96407a	204481a
Amplicon size (bp)	88	156	331
Amplification efficiency (AE)	1.04b	1.04c	N/A
r2	0.994b	0.994c	N/A
Error	0.442b	0.247c	N/A

^aCalculated at http://www.bioinformatics.org/sms2/dna_mw.html, accessed 2018-09-27.

^bCalculated from standard curve with 6 dilutions ranging from 5 × 10⁻⁶–5 × 10⁻¹ ng/μL.

^cCalculated from standard curve with 5 dilutions ranging from 0.04 to 25 ng/μL.