Table 2.

Investigation of the possible thermal degradation or inactivation of reagents. DNA polymerase, nucleotides or primers were subjected to PCR cycling for 30, 45 or 60 cycles, prior to the actual PCR analysis. Data are presented as ΔCq ± standard deviation (n = 3). ΔCq = Cq(analysis with pre-cycled reagent) - Cq(control). ΔCq values above zero indicate partially impaired amplification.

Nr. of PCR cycles performed to test thermal stability	Ex Taq HS	Taq polymerase	dNTPs	Primers
30 PCR cycles	−3.05 ± 0.11	−0.24 ± 0.21	−0.01 ± 0.06	0.00 ± 0.03
45 PCR cycles	−1.81 ± 0.22	0.22 ± 0.05	0.02 ± 0.02	0.00 ± 0.04
60 PCR cycles	−1.75 ± 0.21	−0.03 ± 0.04	0.05 ± 0.11	0.11 ± 0.11