Table 4.

Addition of non-target genomic DNA to a qPCR assay. The RB1 assay was applied, with Ex Taq HS and Taq polymerase. Results are presented as ΔCq ± standard deviation (n = 3). ΔCq = Cq(addition of non-target gDNA) - Cq(control). ΔCq values above zero indicate partially impaired amplification.

	Amount of non-target genomic DNA
	0.5 μg	1 μg	2.5 μg	5 μg	10 μg
Ex Taq HS	−0.08 ± 0.02	−0.16 ± 0.04	−0.26 ± 0.01	−0.38 ± 0.05	−0.43 ± 0.08
Taq polymerase	−0.27 ± 0.06	−0.31 ± 0.04	−0.23 ± 0.10	0.15 ± 0.16	1.43 ± 0.88