Table 5.

Maximum amplification rates and end-point fluorescence levels for increasing amounts of target amplicons. Data presented as mean values ± standard deviation, for 0.3 or 0.6 μM primers (n = 3). Rfu = relative fluorescence units.

Amount of template amplicons	Maximum amplification rate (dF/dc)		End-point fluorescence (rfu)
	0.3 μM primers	0.6 μM primers	0.3 μM primers	0.6 μM primers
0.0025 μg	0.17 ± 0.013	0.20 ± 0.007	1.6 ± 0.11	2.4 ± 0.11
0.005 μg	0.15 ± 0.007	0.22 ± 0.002	1.4 ± 0.071	2.6 ± 0.067
0.01 μg	0.16 ± 0.012	0.19 ± 0.008	1.4 ± 0.096	2.2 ± 0.072
0.02 μg	0.16 ± 0.011	0.15 ± 0.008	1.4 ± 0.101	1.6 ± 0.11
0.04 μg	0.15 ± 0.006	0.16 ± 0.010	1.3 ± 0.040	1.8 ± 0.12
0.08 μg	0.12 ± 0.006	0.18 ± 0.011	0.93 ± 0.029	2.0 ± 0.12
0.1 μg	0.13 ± 0.006	0.25 ± 0.013	1.0 ± 0.035	2.7 ± 0.18
0.25 μg	0.20 ± 0.007	0.24 ± 0.025	1.4 ± 0.056	2.4 ± 0.19
0.5 μg	0.21 ± 0.005	0.30 ± 0.018	1.4 ± 0.072	3.0 ± 0.056
1 μg	0.15 ± 0.009	0.19 ± 0.014	1.2 ± 0.044	2.2 ± 0.053
2 μg	0.084 ± 0.007	0.13 ± 0.004	1.0 ± 0.043	2.0 ± 0.026
3 μg	0.080 ± 0.005	0.11 ± 0.004	1.2 ± 0.039	2.1 ± 0.046