Fig. 3.

Engineering the promotors of antB and antC to increase the production of deacyl antimycins in S. sp. AL2110. (A) Schematic illustration of promoters engineering in the antimycin gene cluster. Constitutive promoters permE* and kasOp were introduced by pMWCas9 to replace the promoters of antB and antC which are responsible for the transcription of antA (antB has been deleted in AL2110) and antC to antE respectively. (B) Deacyl antimycins (DA-1-5 and DA-10, see Fig. S6) productions in the engineered strain mWHU2487 (I) and wild type AL2110 (II). (C) The production yield of deacyl antimycins in pmWHU2478 and AL2110 during the fermentation period (D2-D5). At the D5, mWHU2487 produces deacyl antimycins (62 mg/L) ∼9 fold higher than AL2110 (6.4 mg/L).