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. 2019 Feb 28;10:221. doi: 10.3389/fpls.2019.00221

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Copyright © 2019 Rípodas, Castaingts, Clúa, Villafañe, Blanco and Zanetti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tissue-specific expression analysis of PvNF-YB7 in roots and nodules of P. vulgaris. Histochemical GUS staining of roots and nodules transformed with the ProPvNF-YB7:GUS construct. (A) Non-inoculated (NI) primary root which containing a lateral root primordia (LRP). (B) Thin section of a primary root containing an emerged lateral root (ELR). (C) Lateral root meristem (LRM) of a NI lateral root. (D) Susceptible zone (SZ) of a NI lateral root. (E) SZ of lateral root inoculated (I) with R. etli strain SC15. Arrows point to infection sites. (F) Nodule primordia (NP) of 6 dpi, (G) developing nodule (DN) of 14 dpi, and (H) mature nodule of 21 dpi formed by strain SC15. NCT: nodule central tissue. Pictures are representative of images observed in more than three independent experiments. Bars: 200 μm.