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. 2019 Feb 28;10:396. doi: 10.3389/fmicb.2019.00396

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Copyright © 2019 Calderón, Tienda, Heredia-Ponce, Arrebola, Cárcamo-Oyarce, Eberl and Cazorla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interphase air–liquid pellicle formation. (A) Pellicle formation of P. chlororaphis PCL1606 and its derivative mutants. Strains were inoculated (final O.D._600nm of 0.08) and grown on TPG media in 24-well plates without agitation at 25°C for 6 days. (B) A similar experiment but using cell-free supernatants of PcPCL1606 mixed with TPG as growth media in order to demonstrate the pellicle recovery in the liquid medium by the defective mutant ΔdarB. (C) The same experiment as B, using GFP-tagged strains to visualize the pellicle using confocal scanning laser microscopy. Size of the bars in the microwell pictures: 5 mm; bars of confocal laser scanning microscopy: 5 μm.