FIGURE 4.

Colony morphology assay and analysis of Congo red binding. P. chlororaphis PCL1606 and its derivative mutants when were grown on (A) 1% TPG agar plates and (B) 1% TPG agar plates containing 40 μg/mL Congo red and 20 μg/mL Coomasssie brilliant blue; (C) a similar experiment but using TPG plate media amended with cell-fee supernatants of PcPCL1606 (1:1) to demonstrate the restoration in colony morphology of the defective mutant ΔdarB. The plates were incubated at 25°C for 5 days. Size of the bar is 5 mm.