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. 2019 Feb 28;10:396. doi: 10.3389/fmicb.2019.00396

Table 1.

Bacterial strains used in this study.

Strain             Relevant characteristicsa Reference
Bacterial strains
Pseudomonas chlororaphis
PCL1606 Wild-type, isolated from Spanish avocado rhizosphere, HPR +++ Cazorla et al., 2006
darA- PCL1606 derivative insertional mutant in darA gene, HPR -, Kmr Calderón et al., 2013
darB- PCL1606 derivative insertional mutant in darB gene, HPR -, Kmr Calderón et al., 2013
darC- PCL1606 derivative insertional mutant in darC gene, HPR +, Kmr Calderón et al., 2013
darS- PCL1606 derivative insertional mutant in darS gene, HPR ++, Kmr Calderón et al., 2013
darR- PCL1606 derivative insertional mutant in darR gene, HPR ++, Kmr Calderón et al., 2013
GacS- PCL1606 derivative insertional mutant in gacS gene, HPR -, Kmr Cazorla et al., 2006
ΔdarB PCL1606 derivative deletional mutant in darB gene, HPR - This study
ComB ΔdarB transformed with the plasmid pCOMB. HPR +++, Gmr and Kmr This study
Fungal strains
Fusarium oxysporum f. sp. radicis-lycopersici
ZUM2407 Causes crown and foot rot of tomato IPO-DLO Wageningen, The Netherlands
Rosellinia necatrix
CH53 Wild-type, isolated from avocado root rot, High virulence Pérez-Jiménez, 1997
Plasmids
pCOMB darB gene cloned into pBBR1MCS-5 used for complementing mutation on strain ΔdarB, Gmr Calderón et al., 2013
pBAH8 pBBR1MCS-5-containing PA1/04/03-gfp mut3-To-T1; Gmr Huber et al., 2002
pGEM®-T Easy Vector Linearized vector with single 3’-terminal thymidine at both ends Promega

aHPR: Production of 2-hexyl, 5-propyl resorcinol detected by thin-layer chromatography plates (Cazorla et al., 2006). +++ = HPR production level of the wild-type strain P. chlororaphis PCL1606; ++ = ½ of HPR production; + = ¼ of HPR production; - = no production (Calderón et al., 2014a). Antibiotic resistance: Kmr = Kanamycin, Gmr = Gentamycin. IPO-DLO: Institute for Plant Protection – Agriculture Research Department.