Table 1.
Strain Relevant characteristicsa | Reference | ||
---|---|---|---|
Bacterial strains | |||
Pseudomonas chlororaphis | |||
PCL1606 | Wild-type, isolated from Spanish avocado rhizosphere, HPR +++ | Cazorla et al., 2006 | |
darA- | PCL1606 derivative insertional mutant in darA gene, HPR -, Kmr | Calderón et al., 2013 | |
darB- | PCL1606 derivative insertional mutant in darB gene, HPR -, Kmr | Calderón et al., 2013 | |
darC- | PCL1606 derivative insertional mutant in darC gene, HPR +, Kmr | Calderón et al., 2013 | |
darS- | PCL1606 derivative insertional mutant in darS gene, HPR ++, Kmr | Calderón et al., 2013 | |
darR- | PCL1606 derivative insertional mutant in darR gene, HPR ++, Kmr | Calderón et al., 2013 | |
GacS- | PCL1606 derivative insertional mutant in gacS gene, HPR -, Kmr | Cazorla et al., 2006 | |
ΔdarB | PCL1606 derivative deletional mutant in darB gene, HPR - | This study | |
ComB | ΔdarB transformed with the plasmid pCOMB. HPR +++, Gmr and Kmr | This study | |
Fungal strains | |||
Fusarium oxysporum f. sp. radicis-lycopersici | |||
ZUM2407 | Causes crown and foot rot of tomato | IPO-DLO Wageningen, The Netherlands | |
Rosellinia necatrix | |||
CH53 | Wild-type, isolated from avocado root rot, High virulence | Pérez-Jiménez, 1997 | |
Plasmids | |||
pCOMB | darB gene cloned into pBBR1MCS-5 used for complementing mutation on strain ΔdarB, Gmr | Calderón et al., 2013 | |
pBAH8 | pBBR1MCS-5-containing PA1/04/03-gfp mut3-To-T1; Gmr | Huber et al., 2002 | |
pGEM®-T Easy Vector | Linearized vector with single 3’-terminal thymidine at both ends | Promega | |
aHPR: Production of 2-hexyl, 5-propyl resorcinol detected by thin-layer chromatography plates (Cazorla et al., 2006). +++ = HPR production level of the wild-type strain P. chlororaphis PCL1606; ++ = ½ of HPR production; + = ¼ of HPR production; - = no production (Calderón et al., 2014a). Antibiotic resistance: Kmr = Kanamycin, Gmr = Gentamycin. IPO-DLO: Institute for Plant Protection – Agriculture Research Department.