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. 2019 Feb 28;10:118. doi: 10.3389/fpls.2019.00118

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Copyright © 2019 Gautier, Label, Eliášová, Leplé, Motyka, Boizot, Vondráková, Malbeck, Trávníčková, Le Metté, Lesage-Descauses, Lomenech, Trontin, Costa, Lelu-Walter and Teyssier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The concentrations of isoprenoid cytokinins (CKs; A), aromatic CKs (B) and hydroxylated N⁶-benzyladenine (BA) derivatives (C) in isogenic embryonal mass (EM) and non-embryogenic callus (NEC) for three genotypes (SD4-8, TD15-1, TD17-1) of Douglas-fir at the proliferation phase of somatic embryogenesis. The following isoprenoid and aromatic CK derivatives were detected: CK free bases: N⁶-(Δ²-isopentenyl)adenine. CK ribosides: trans-zeatin 9-riboside, cis-zeatin 9-riboside, N⁶-(Δ²-isopentenyl)adenosine. CK nucleotides: cis-zeatin 9-riboside-5’-monophosphate, N⁶-(Δ²-isopentenyl)adenosine-5^′-monophosphate, dihydrozeatin 9-riboside-5^′-monophosphate. CK O-glucosides: trans-zeatin O-glucoside, cis-zeatin 9-riboside O-glucoside. CK N-glucosides: trans-zeatin-N7-glucoside, cis-zeatin-N7-glucoside, dihydrozeatin-N7-glucoside. BA: N⁶-benzyladenine. BA riboside: BA-9-riboside. BA nucleotide: BA-9-riboside-5^′- monophosphate. BA N-glucosides: BA-N3-glucoside, BA-N7-glucoside, BA-N9-glucoside. OH-BA: para-hydroxy-BA, meta-hydroxy-BA, ortho-hydroxy-BA. OH-BA ribosides: para-hydroxy-BA-9-riboside, meta-hydroxy-BA-9-riboside. OH-BA nucleotides: para-hydroxy-BA-9-riboside-5^′-monophosphate, meta-hydroxy-BA-9-riboside-monophosphate, ortho-hydroxy-BA-9-riboside- monophosphate. OH-BA N-glucosides: meta-hydroxy-BA-N9-glucoside, ortho-hydroxy-BA-N9-glucoside.