Table 1.
Soluble sugar, starch and soluble protein contents of isogenic embryonal mass (EM) and non-embryogenic callus (NEC) of Douglas fir after 2 weeks of multiplication: means obtained from analyses of three genotypes (SD4-8, TD15-1, and TD17-1), n = 4 for carbohydrate and starch analyses and 5 for soluble protein determinations (in each case ±SE).
| Compounds | EM | NEC |
|---|---|---|
| Carbohydrate content (μg g-1 d.w.) | ||
| Fructose (Fru) | 17.14 ± 4.27 ab | 10.78 ± 12.80 ab |
| Galactose | 2.68 ± 0.77 a | 0.00 a |
| Glucose (Glc) | 208.40 ± 32.74 d | 90.79 ± 76.97 c |
| Sucrose (Suc) | 41.23 ± 19.53 b | 26.87 ± 12.93 ab |
| Maltose | 6.09 ± 0.76 a | 8.95 ± 12.53 a |
| Melibiose | 3.46 ± 0.82 a | 0.00 a |
| Myo-inositol | 3.30 ± 0.82 a | 4.03 ± 0.66 a |
| Raffinose | 11.58 ± 5.12 ab | 5.33 ± 3.92 a |
| Carbohydrate total | 869.47 ± 70.00 e | 440.25 ± 43.32 f |
| [(Glc + Fru)/Suc] | 7.50 ± 1.49 * | 3.14 ± 0.59 ** |
| Starch (mg g-1 d.w.) | 9.03 ± 0 0.89 α | 19.01 ± 1.37 β |
| Soluble proteins (mg g-1 d.w.) | 94.19 ± 7 0.54 A | 92.27 ± 27.85 A |
Superscript letters and asterisks indicate significance differences between EM and NEC according to multiple comparison of means (P < 0.05).