Table 2.

Main microbiota (accounting for ≥0.05% of the total sequences in at least one of the samples) that significantly changed between saponins treated group and control (abundance of the genera is expressed as a percentage).

	Genus	Treatments
Phyluma		Control	Saponin-treated	SEM	P-values	FDRb
AH vs. AHS
Firmicutes	Ruminococcaceae_NK4A214	1.97	0.91	0.352	0.04	0.63
	Ruminococcaceae_UCG-008	0.20	0.08	0.032	<0.01	0.52
	Lachnospiraceae_NK3A20p	0.80	0.33	0.138	0.05	0.63
Bacteroidetes	Prevotellaceae_YAB2003	0.06	0.17	0.025	<0.01	0.50
Spirochaetae	Sphaerochaeta	0.02	0.06	0.013	0.05	0.63
SH vs. SHS
Firmicutes	Lachnospiraceae_NA	7.03	4.56	0.583	0.01	0.09
	Lachnospiraceae_NK3A20	2.16	0.69	0.353	0.02	0.10
	Erysipelotrichaceae_UCG-002	1.34	0.59	0.227	0.04	0.19
	Ruminococcaceae_NK4A214	0.52	0.27	0.051	<0.01	0.05
	Ruminococcaceae_UCG-002	0.04	0.11	0.010	<0.01	0.02
	Syntrophococcus	0.45	0.24	0.030	<0.01	<0.01
	Pseudoramibacter	0.11	0.07	0.013	0.03	0.14
	Christensenellaceae_R-7	0.12	0.25	0.025	<0.01	0.05
	Clostridium_sensu_stricto_1	0.01	0.14	0.012	<0.01	<0.01
Bacteroidetes	Prevotella_1	0.60	4.32	0.259	<0.01	<0.01
	Prevotellaceae_Ga6A1	0.06	0.23	0.021	<0.01	<0.01
	Prevotellaceae_UCG-001	0.07	0.12	0.012	0.01	0.09
	Prevotellaceae_YAB2003	0.003	0.07	0.003	<0.01	<0.01
Spirochaetae	Treponema_2	0.16	0.50	0.103	0.04	0.21

^aAH, alfalfa hay diet; AHS, saponins treated AH; SH, soybean hull diet; SHS, saponins treated SH. ^bFDR, false discovery rate.